HeLa Cell Culture

HeLa Cell Culture

Cell Biology
All Specimens

Cultured HeLa cells (cervical adenocarcinoma) in monolayer

Stains: unstained fluorescence he
Images: 3
Compatible Scopes: 9
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3 images
HeLa Cells — Hoechst Fluorescence Stain
HeLa Cells — Hoechst Fluorescence Stain

HeLa cervical carcinoma cells stained with Hoechst 33258 nuclear dye. Nuclei fluoresce bright blue against a dark background, revealing nuclear morphology, mitotic figures, and chromatin condensation patterns.

fluorescence Downloaded 2026-03-16
Fluorescence All In One
40× Fluorescence Focus 0.5 NA 1.25

Hoechst 33258 — DNA-binding fluorescent dye highlighting nuclei

Wikimedia Commons — CC BY-SA 3.0

HeLa Cell Monolayer — Phase Contrast
HeLa Cell Monolayer — Phase Contrast

Phase contrast microscopy image of HeLa cells in culture showing the characteristic epithelial-like monolayer morphology. Cells are adherent with visible cell boundaries and refractile cytoplasm.

unstained Downloaded 2026-03-16
Inverted Optical
20× Phase Contrast Focus 0.5 NA 0.40

Phase contrast — unstained living cells in culture flask

Wikimedia Commons — CC BY 3.0

HeLa Cells — Culture Morphology
HeLa Cells — Culture Morphology

Inverted microscopy image of HeLa cells growing in monolayer culture. The cells show typical epithelioid morphology with irregular polygonal shapes and prominent nuclei visible in phase contrast.

unstained Downloaded 2026-03-16
Inverted Optical
10× Phase Contrast Focus 0.5 NA 0.25

Standard HeLa cell line morphology in culture

Wikimedia Commons — CC BY 3.0

Compatible Microscopes

Model Manufacturer Type Magnification Range NA Max Resolution
BZ-X810 Keyence Fluorescence All In One 2–100× 1.45 190 nm
DMi8 Leica Microsystems Inverted Optical 2.5–100× 1.47 185 nm
STELLARIS 5 Leica Microsystems Confocal 5–100× — 120 nm
Eclipse Ti2 Nikon Inverted Optical 2–100× 1.45 190 nm
A1R HD25 Nikon Confocal 4–100× — 140 nm
IX83 Olympus (Evident) Inverted Optical 2–100× 1.4 200 nm
FV4000 Olympus (Evident) Confocal 4–100× — 120 nm
Axio Observer 7 Zeiss Inverted Optical 5–100× 1.4 200 nm
LSM 980 Zeiss Confocal 5–63× — 120 nm